Evaluation of milk yield losses associated with Salmonella antibodies in bulk-tank milk in bovine dairy herds

The effect of Salmonella on milk production is not well established in cattle. The objective of this study was to investigate whether introduction of Salmonella into dairy cattle herds was associated with reduced milk yield and the duration of any effect. Longitudinal data from 2005 through 2009 were used, with data from 12 months before until 18 months after the estimated date of infection. Twenty-eight case herds were selected based on an increase in the level of Salmonella specific antibodies in bulk-tank milk from < 10 corrected optic density percentage (ODC%) to ≥ 70 ODC% between two consecutive 3-monthly measurements in the Danish Salmonella surveillance program. All selected case herds were conventional Danish Holstein herds. Control herds (n = 40) were selected randomly from Danish Holstein herds with Salmonella antibody levels consistently < 10 ODC%. A date of herd infection was randomly allocated to the control herds. Hierarchical mixed effect models with the outcome test day energy corrected milk yield (ECM)/cow were used to investigate the daily milk yield before and after the estimated herd infection date for cows in parity 1, 2 and 3+. Control herds were used to evaluate whether the effects in the case herds could be reproduced in herds without Salmonella infection. Herd size, days in milk, somatic cell count, season, and year were included in the models. The key results were that first parity cow yield was reduced by a mean of 1.4 kg (95% CI: 0.5 to 2.3) ECM/cow per day from seven to 15 months after the estimated herd infection date, compared with first parity cows in the same herds in the 12 months before the estimated herd infection date. Yield for parity 3+ was reduced by a mean of 3.0 kg (95% CI: 1.3 to 4.8) ECM/cow per day from seven to 15 months after herd infection compared with parity 3+ cows in the 12 months before the estimated herd infection. There were minor differences in yield in second parity cows before and after herd infection, and no difference between cows in control herds before and after the simulated infection date. There was a significant drop in milk yield in affected herds and the reduction was detectable several months after the increase in bulk-tank milk Salmonella antibodies. It took more than a year for milk yield to return to pre- infection levels

Continuous monitoring of aerial bioburden within intensive care isolation rooms and identification of 'high risk' activities

Background: The spread of pathogens via the airborne route is often underestimated and little is known about the extent to which airborne microbial contamination levels vary throughout the day and night in hospital facilities. Aims: This study aims to evaluate variability in airborne contamination levels within ICU isolation rooms over extended time periods to improve understanding of the extent to which ward activities, and consequential increases in airborne bioburden, may contribute to cross-infection of patients. Methods: Environmental air monitoring was conducted within occupied and vacant inpatient isolation rooms. A sieve impactor sampler was used to collect 500 L air samples every 15 minutes over 10-hour (08:00-18:00 h) and 24-hour (08:00-08:00 h) periods. Samples were collected, room activity logged, and the bacterial contamination levels were recorded as cfu/m3 of air. Findings: A high degree of variability in levels of airborne contamination was observed across all scenarios in the studied isolation rooms. Air bioburden increased as room occupancy increased, with air contamination levels highest in rooms occupied for the longest time during the study (10 days) with a mean value of 104.4 cfu/m3 and a range of 12–510 cfu/m3. Counts were lowest in unoccupied rooms, with an average value of 20 cfu/m3 and during the night. Conclusion: Peaks in airborne contamination showed a direct relation to an increase in activity levels. This study provides first clear evidence of the extent of variability in microbial airborne levels over 24-hour periods in ICU isolation rooms and directly correlates microbial load to ward activity

Numerical Modeling of Flow Control in a Boundary-Layer-Ingesting Offset Inlet Diffuser at Transonic Mach Numbers

This paper will investigate the validation of the NASA developed, Reynolds-averaged Navier-Stokes (RANS) flow solver, OVERFLOW, for a boundary-layer-ingesting (BLI) offset (S-shaped) inlet in transonic flow with passive and active flow control devices as well as a baseline case. Numerical simulations are compared to wind tunnel results of a BLI inlet experiment conducted at the NASA Langley 0.3-Meter Transonic Cryogenic Tunnel. Comparisons of inlet flow distortion, pressure recovery, and inlet wall pressures are performed. The numerical simulations are compared to the BLI inlet data at a free-stream Mach number of 0.85 and a Reynolds number of approximately 2 million based on the fanface diameter. The numerical simulations with and without tunnel walls are performed, quantifying tunnel wall effects on the BLI inlet flow. A comparison is made between the numerical simulations and the BLI inlet experiment for the baseline and VG vane cases at various inlet mass flow rates. A comparison is also made to a BLI inlet jet configuration for varying actuator mass flow rates at a fixed inlet mass flow rate. Overall, the numerical simulations were able to predict the baseline circumferential flow distortion, DPCP avg, very well within the designed operating range of the BLI inlet. A comparison of the average total pressure recovery showed that the simulations were able to predict the trends but had a negative 0.01 offset when compared to the experimental levels. Numerical simulations of the baseline inlet flow also showed good agreement with the experimental inlet centerline surface pressures. The vane case showed that the CFD predicted the correct trends in the circumferential distortion levels for varying inlet mass flow but had a distortion level that was nearly twice as large as the experiment. Comparison to circumferential distortion measurements for a 15 deg clocked 40 probe rake indicated that the circumferential distortion levels are very sensitive to the symmetry of the flow and that a misalignment of the vanes in the experiment could have resulted in this difference. The numerical simulations of the BLI inlet with jets showed good agreement with the circumferential inlet distortion levels for a range of jet actuator mass flow ratios at a fixed inlet mass flow rate. The CFD simulations for the jet case also predicted an average total pressure recovery offset that was 0.01 lower than the experiment as was seen in the baseline. Comparisons of the flow features for the jet cases revealed that the CFD predicted a much larger vortex at the engine fan-face when compare to the experiment

Weak Localization Effect in Superconductors by Radiation Damage

Large reductions of the superconducting transition temperature $T_{c}$ and the accompanying loss of the thermal electrical resistivity (electron-phonon interaction) due to radiation damage have been observed for several A15 compounds, Chevrel phase and Ternary superconductors, and $\rm{NbSe_{2}}$ in the high fluence regime. We examine these behaviors based on the recent theory of weak localization effect in superconductors. We find a good fitting to the experimental data. In particular, weak localization correction to the phonon-mediated interaction is derived from the density correlation function. It is shown that weak localization has a strong influence on both the phonon-mediated interaction and the electron-phonon interaction, which leads to the universal correlation of $T_{c}$ and resistance ratio.Comment: 16 pages plus 3 figures, revtex, 76 references, For more information, Plesse see http://www.fen.bilkent.edu.tr/~yjki

Does nature conservation enhance ecosystem services delivery?

Whilst a number of studies have examined the effects of biodiversity conservation on the delivery of ecosystems, they have been often limited by the scope of the ecosystem services (ES) assessed and often suffer from confounding spatial issues. This paper examines the impacts of nature conservation (designation) on the delivery of a full suite of ES across nine case-studies in the UK, using expert opinion. The case-studies covered a range of habitats and explore the delivery of ES from a ‘protected site’ and a comparable ‘non-protected’ site. By conducting pair-wise comparisons between comparable sites our study is one of the first to attempt to mitigate confounding cause and effect factors in relation to spatial context in correlative studies. Protected sites delivered higher levels of ecosystem services than nonprotected sites, with the main differences being in the cultural and regulating ecosystem services. Against expectations, there was no consistent negative impact of protection on provisioning services across the case-studies. Whilst the analysis demonstrated general patterns and differences in ecosystem delivery between protected and non-protected sites, the individual responses in each case-study highlights the importance of the social, biophysical, economic and temporal context of individual protected areas and the associated management

Species abundance dynamics under neutral assumptions: a Bayesian approach to the controversy

1. Hubbell's 'Unified Neutral Theory of Biodiversity and Biogeography' (UNTB) has generated much controversy about both the realism of its assumptions and how well it describes the species abundance dynamics in real communities. 2. We fit a discrete-time version of Hubbell's neutral model to long-term macro-moth (Lepidoptera) community data from the Rothamsted Insect Survey (RIS) light-traps network in the United Kingdom. 3. We relax the assumption of constant community size and use a hierarchical Bayesian approach to show that the model does not fit the data well as it would need parameter values that are impossible. 4. This is because the ecological communities fluctuate more than expected under neutrality. 5. The model, as presented here, can be extended to include environmental stochasticity, density-dependence, or changes in population sizes that are correlated between different species

Transverse spin dynamics in a spin-polarized Fermi liquid

The linear equations for transverse spin dynamics in weakly polarised degenerate Fermi liquid with arbitrary relationship between temperature and polarization are derived from Landau-Silin phenomenological kinetic equation with general form of two-particle collision integral. The temperature and polarization dependence of the spin current relaxation time is established. It is found in particular that at finite polarization transverse spin wave damping has a finite value at T=0. The analogy between temperature dependences of spin waves attenuation and ultrasound absorption in degenerate Fermi liquid at arbitrary temperature is presented. We also discuss spin-polarized Fermi liquid in the general context of the Fermi-liquid theory and compare it with "Fermi liquid" with spontaneous magnetization.Comment: 10 page

Establishment and optimization of genomic selection to accelerate the domestication and improvement of intermediate wheatgrass

Citation: Zhang, X., Sallam, A., Gao, L., Kantarski, T., Poland, J., DeHaan, L. R., . . . Anderson, J. A. (2016). Establishment and optimization of genomic selection to accelerate the domestication and improvement of intermediate wheatgrass. Plant Genome, 9(1). doi:10.3835/plantgenome2015.07.0059Intermediate wheatgrass (IWG) is a perennial species and has edible and nutritious grain and desirable agronomic traits, including large seed size, high grain yield, and biomass. It also has the potential to provide ecosystem services and an economic return to farmers. However, because of its allohexaploidy and self-incompatibility, developing molecular markers for genetic analysis and molecular breeding has been challenging. In the present study, using genotyping-by-sequencing (GBS) technology, 3436 genomewide markers discovered in a biparental population with 178 genets, were mapped to 21 linkage groups (LG) corresponding to 21 chromosomes of IWG. Genomic prediction models were developed using 3883 markers discovered in a breeding population containing 1126 representative genets from 58 half-sib families. High predictive ability was observed for seven agronomic traits using cross-validation, ranging from 0.46 for biomass to 0.67 for seed weight. Optimization results indicated that 8 to 10 genets from each half-sib family can form a good training population to predict the breeding value of their siblings, and 1600 genomewide markers are adequate to capture the genetic variation in the current breeding population for genomic selection. Thus, with the advances in sequencing-based marker technologies, it was practical to perform molecular genetic analysis and molecular breeding on a new and challenging species like IWG, and genomic selection could increase the efficiency of recurrent selection and accelerate the domestication and improvement of IWG.A. © Crop Science Society of America

Whole exome sequence analysis reveals a homozygous mutation in PNPLA2 as the cause of severe dilated cardiomyopathy secondary to neutral lipid storage disease.

Accepted manuscript 12 month embargo, pre-print immediately